PAMPA allpeptides

Use this form to generate all tryptic peptides—or any digested peptides—from a set of sequences and build a peptide table. Optionally, you can filter the peptides to retain only those whose theoretical masses are detectable in a selected set of mass spectra. More...


Name of the job (optional) :

Target sequences

Upload the FASTA files for your species of interest.

Upload a limit file (optional, doc : https://github.com/touzet/pampa/wiki/Limiting-searches).

Parameters for in silico digestion

Digestion enzyme
Maximal peptide length
Minimal peptide length
Maximal number of missed cleavage sites

Mass spectra (optional) :

Upload your MS spectra files in CSV, MGF or mzML format (or in a .ZIP archive)

Mass error tolerance
Optimize for MALDI-TOF spectra (50.0 ppm)
Optimize for MALDI-FTICR spectra: (5.0 ppm)
Custom value in ppm
Custom value in Daltons

Minimal proportion of matching peaks (between 0 and 1)